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the International Space Station is used

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by all of the partners space agencies

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for scientific research that can't be

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done in an environment where gravity is

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dominant in the case of some medical

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experiments the weightlessness allows

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cells to behave in ways that help

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researchers learn about the nature of

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cancer cells which is information that

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can help in the fight against the

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disease one recent example of that is an

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experiment known as cell box thyroid

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cancer and to tell us about it we're

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joined this morning by the principal

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investigator dr. daniela grimm of the

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department of biomedicine pharmacology

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at our house university in denmark and

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her colleague dr. Marcus villain of the

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University of magdeburg dr. Grimm you

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had a previous study on the station in

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which you found that tumor cells behave

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a little less aggressively in space do

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we know yet why that is we use a fully

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differentiated thyroid cancer cell line

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which is a very aggressive two more this

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tumor cell line comes from a lymph node

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metastases from a 42 year old male and

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this experiment which we had on a

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spaceflight before cell box showed that

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these tener cancer cells produces a lot

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of genes and proteins which were

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regulated in a way that it seems to that

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they promote to shift to a less

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malignant form we don't know what is the

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reason for it because it was first time

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done and it was an empiric observation

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first but we have found that these cells

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grow three-dimensionally that means like

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a ball and these are round egg regards

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we call them so roads which are very

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similar to a metastasis in the blood or

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micro to muah they are nearer to the in

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vivo situation and the primary focus of

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the box was that we wanted to repeat the

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experiments and further elucidate the

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underlying mechanisms so that was with

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you

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to build on with this current experiment

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yeah this was the main purpose of the

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cbox experiment was to reproduce the

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results which we had obtained from the

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former space experiment and another

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important point is that observed effects

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we have to exclude that they are not

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artifact and we have to reproduce them

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one important point was that we wanted

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to focus on the proteins which may be

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involved in 3d close formation to which

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I involved in thyroid formation and

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during the side-box mission we have

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found interesting proteins which might

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play a role in the inhibition of this

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really grows and such proteins are

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related to a matrix around the cells it

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called extracellular matrix where a lot

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of signaling between the cells takes

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place and currently we are working on

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secreted proteins and have some interest

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interested data which we will publish in

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the near future your experiment flew to

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the station on a dragon cargo ship about

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18 months ago tell us about what samples

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it was that you use that you sent to

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space and what was done with them while

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they were there on the space station

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this was a cell line thyroid cancer cell

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encode FTC 133 which she studied in

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detail near the last ten years and it's

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well known in the scientific research

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area the cells were seated in the

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side-box containers and incubated for 37

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for 24 hours in 37 degrees and with

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fastest and co2 overnight on ground in

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the laboratory and the next day the hard

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way was checked by Airbus and the

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engineers then was to hand over to nana

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Rex and NASA nano Rex and NASA people

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transported the containers to the launch

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site and integrated the dragon

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integrated the samples in the Dragon

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spacecraft after their arrival on the

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ice as the hardware

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was installed in the NanoRacks facility

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where the cells were kept at 37 degrees

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and after 10 days the cells were fixed

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with a special fixative you call it on a

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later for later gene analysis in

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addition we collected the supernatant

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the medium and which was analyzed by

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United States company it's called movie

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at IBM in Austin in Texas yeah and after

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when we got everything back from from

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the dragon be analyzed the data and

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you've had the knack for for some time

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now is there is there anything that

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you've seen so far that is encouraging

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to you yes we have had identified

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proteins we have published this also in

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proteomics this year which are involved

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in the negative regulation of thyroid

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formation and in addition they have

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found a variety of biomolecules of

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secreted factors by the cells which were

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found in exerted elevations in space

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samples not on ground samples and many

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of these enzymes which were secreted are

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responsible to degrade these excess

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color matrix proteins what we have found

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also in the proteomic studying and this

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is what we do right now we publish these

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samples these data in the near future

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now it's most experiments on the space

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station involved collaboration from a

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lot of different entities dr. V lon tell

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me about that what it took to get this

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particular experiment in together and

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then get it to orbit and get it back yes

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this was indeed a collaboration of many

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people involved and one of the most

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important roads where of course taken

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back at the drunk Space Agency dlr who

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offered us the opportunity to take part

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in this mission and day of course our

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collaborating with NASA and SpaceX so

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thank you to all those

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companies and institutions who offered

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us the opportunity then there was elbows

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defense in space who designed and

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constructed the cell culturing

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hardware's to our specifications space

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laboratories space life sciences

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laboratory is provided as a laboratory

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space in kc where we could prepare for

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both flight culture the sales and of

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course see the harbor where then nana

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legs of the infrastructure on board the

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ISS where all the sandbox happen could

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be housed and incubated at the right

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temperature and of course finally and

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last but not least we would also like to

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extend our thanks to our universities of

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our math book always supported our

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research dr. Grimm are you planning for

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for future experiments to try to follow

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up on what you've learned so far yes we

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have one space x8 the ESS were always

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project today they had a teleconference

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and we heard that the prospected launch

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date would be in January three early

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this next year on this mission we will

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have endothelial cells and we will focus

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on three dimensional growth and we will

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also see whether some growth factors can

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induce to formation the author will

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focus on several mechanisms of cell

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death and of differentiation and the

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salutation changes of these sounds dr.

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Graham gah dr. Wilander good luck with

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your work hope you have success as you

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continue dr. daniela grim of the is the

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principal investigator of the cell box